The arctical says that this happens after the 100 chapter in the Manga. But I just read the Manga and in the circus arc, after Snake is left alone in the circus and he goes to search for Joker and co. he leaves the circus saying something like "its so lonely" (I only own the german manga so I cant exactly say what he says in the englisch or original version) and he says it, there is no adding with a snake. So, that would have been the first time he talks without his snakes?

please correct me if im wrong, im very curious.